Peltier jụrụ oyi (teknụzụ jụrụ oyi na-ekpo ọkụ na-adabere na mmetụta Peltier) aghọwo otu n'ime teknụzụ bụ isi nke sistemu njikwa okpomọkụ maka ngwa PCR (polymerase chain reaction) n'ihi mmeghachi omume ngwa ngwa ya, njikwa okpomọkụ nke ọma, na nha kọmpat, na-emetụta arụmọrụ, izi ezi na ngwa ngwa nke PCR. Ihe na-esonụ bụ nkọwa zuru ezu nke ngwa na uru dị na thermoelectric jụrụ (peltier cooling) malite na isi ihe PCR chọrọ:
I. Isi ihe achọrọ maka njikwa okpomọkụ na teknụzụ PCR
The isi usoro nke PCR bụ a ugboro ugboro okirikiri denaturation (90-95 ℃), annealing (50-60 ℃), na ndọtị (72 ℃), nke nwere nnọọ nlezianya chọrọ maka okpomọkụ akara usoro.
Okpomọkụ na-ebili ma daa: Mbelata oge nke otu okirikiri (dịka ọmụmaatụ, ọ na-ewe naanị sekọnd ole na ole iji dobe site na 95 ℃ ruo 55 ℃), ma kwalite nrụpụta mmeghachi omume;
High-nkenke okpomọkụ akara: A deviation nke ± 0.5 ℃ na annealing okpomọkụ nwere ike iduga na-abụghị kpọmkwem amplification, na ọ ga-achịkwa n'ime ± 0.1 ℃.
Ịdị n'otu ọnọdụ okpomọkụ: Mgbe ọtụtụ nlele na-emeghachi n'otu oge, ọdịiche okpomọkụ dị n'etiti sample Wells kwesịrị ịbụ ≤0.5 ℃ iji zere ngbanwe nsonaazụ.
Mgbanwe nke ntakiri: PCR nwere ike ibugharị (dị ka nlele saịtị POCT scenarios) kwesịrị ịdị kọmpat n'ogo na enweghị akụkụ nke eji arụ ọrụ.
II. Ngwa isi nke ikuku ọkụ na PCR
The thermoelectric Cooler TEC, Thermoelectric jụrụ oyi modul, peltier modul na-enweta "bidirectional mgbanwe nke kpo oku na jụrụ" site kpọmkwem ugbu a, n'ụzọ zuru okè dakọtara okpomọkụ chọrọ PCR. Ngwa ya akọwapụtara na-egosipụta n'akụkụ ndị a:
1. Mbilite na ọdịda okpomọkụ ngwa ngwa: belata oge mmeghachi omume
Ụkpụrụ: Site n'ịgbanwe ntụziaka nke ugbu a, TEC modul, thermoelectric modul, peltier ngwaọrụ nwere ike gbanwee ngwa ngwa n'etiti "ọkụ" (mgbe ugbu a na-aga n'ihu, njedebe okpomọkụ nke TEC modul , modul peltier na-aghọ njedebe na-ahapụ okpomọkụ) na "iju oyi" (mgbe ugbu a na-atụgharị, nkwụsị ọkụ na-ewepụta oge, nkwụsị na-emekarị ka ọ bụrụ ihe na-eme ka ọ bụrụ ihe na-erughị 1) nke abụọ.
Uru: Ụzọ nju oyi nke ọdịnala (dị ka fan na compressors) na-adabere na ntụzịaka okpomọkụ ma ọ bụ mmegharị igwe, ọnụego kpo oku na nju oyi na-abụkarị ihe na-erughị 2℃/s. Mgbe TEC jikọtara ya na ihe mgbochi metal dị elu (dịka ọla kọpa na aluminom alloy), ọ nwere ike nweta ọkụ ọkụ na nju oyi nke 5-10 ℃ / s, na-ebelata oge okirikiri PCR site na nkeji 30 ruo ihe na-erughị nkeji 10 (dị ka ngwa PCR ngwa ngwa).
2. Njikwa okpomọkụ dị elu nke ọma: Na-eme ka nkọwapụta nkwalite
Ụkpụrụ: Ike mmepụta (ọkụ ọkụ / ikpo ọkụ) nke TEC modul, modul jụrụ oyi, modul thermoelectric na-ejikọta ya na ike dị ugbu a. Ejikọtara ya na ihe mmetụta okpomọkụ dị elu (dị ka nguzogide platinum, thermocouple) na usoro nchịkwa nzaghachi PID, enwere ike gbanwee nke dị ugbu a ozugbo iji nweta njikwa okpomọkụ.
Uru: The okpomọkụ akara ziri ezi nwere ike iru ± 0.1 ℃, nke dị nnọọ elu karịa nke omenala mmiri mmiri bath ma ọ bụ compressor refrigeration (± 0.5 ℃). Dị ka ọmụmaatụ, ọ bụrụ na ezubere iche okpomọkụ n'oge annealing ogbo bụ 58 ℃, TEC modul, thermoelectric modul, peltier ngwa nju oyi, peltier element nwere ike kwụsie ike na-ejigide okpomọkụ a, na-ezere na-abụghị kpọmkwem njikọ nke primers n'ihi okpomọkụ mgbanwe na budata na-eme ka nkwalite nkọwapụta.
3. Nhazi dị ntakịrị: Na-akwalite mmepe nke PCR na-ebugharị
Ụkpụrụ: The olu nke TEC modul, peltier element, peltier ngwaọrụ bụ nanị ole na ole square centimeters (dịka ọmụmaatụ, a 10 × 10mm TEC modul, thermoelectric jụrụ modul, peltier modul nwere ike izute chọrọ nke otu sample), ọ dịghị n'ibu na-akpụ akpụ akụkụ (dị ka piston nke compressor ma ọ bụ fan agụba), na ọ dịghị achọ refrigerant.
Uru: Mgbe ngwa PCR ọdịnala na-adabere na compressors maka jụrụ oyi, olu ha na-adịkarị karịa 50L. Otú ọ dị, ngwá ọrụ PCR na-ebugharị na-eji thermoelectric jụrụ oyi, modul thermoelectric, peltier modul, TEC modul nwere ike ibelata ihe na-erughị 5L (dị ka ngwaọrụ ejiri aka), na-eme ka ha dị mma maka ule ubi (dị ka nyocha na saịtị n'oge ọrịa na-efe efe), nyocha ụlọ ọgwụ, na ọnọdụ ndị ọzọ.
4. Ịdị n'otu okpomọkụ: Gbaa mbọ hụ na ịdị n'otu n'etiti ụdị dị iche iche
Ụkpụrụ: Site n'ịhazi ọtụtụ nhazi nke TEC arrays (dị ka 96 micro TECs kwekọrọ na efere 96-well), ma ọ bụ na-ejikọta ya na ikpo ọkụ metal blocks (elu thermal conductivity ihe), mgbanwe okpomọkụ kpatara site na onye dị iche na TEC nwere ike ịkwụsị.
Uru: Enwere ike ịchịkwa ọdịiche okpomọkụ dị n'etiti sample Wells n'ime ± 0.3 ℃, na-ezere ọdịiche arụmọrụ arụmọrụ kpatara site na okpomọkụ na-ekwekọghị ekwekọ n'etiti ọnụ Wells na Central Wells, yana hụ na nha nke nsonaazụ nlele (dị ka nkwekọ nke ụkpụrụ CT na PCR nke oge fluorescence quantitative).
5. Ịtụkwasị obi na ịnọgide na-enwe ike: Belata ụgwọ ọrụ ogologo oge
Ụkpụrụ: TEC enweghị akụkụ iyi, nwere ndụ ihe karịrị 100,000 awa, ọ dịghịkwa achọ ngbanwe nke refrigerant mgbe niile (dị ka Freon na compressors).
Uru: Nkezi ndụ ngwa PCR nke igwe mkpakọ ọdịnala na-ajụ oyi bụ ihe dịka afọ 5 ruo 8, ebe sistemụ TEC nwere ike ịgbatị ya karịa afọ 10. Ọzọkwa, mmezi naanị chọrọ ihicha ọkụ ọkụ, nke ukwuu na-ebelata ọrụ na ụgwọ ọrụ nke akụrụngwa.
III. Ihe ịma aka na nkwalite dị na ngwa
Semiconductor jụrụ oyi ezughị oke na PCR ma chọọ nkwalite ezubere iche:
Okpomọkụ na-ekpochapụ ọkụ: Mgbe TEC na-ajụ oyi, nnukwu okpomọkụ na-akwakọba na njedebe ntọhapụ okpomọkụ (dịka ọmụmaatụ, mgbe okpomọkụ na-ada site na 95 ℃ ruo 55 ℃, okpomọkụ dị iche na-eru 40 ℃, na ike ịhapụ okpomọkụ na-abawanye nke ukwuu). Ọ dị mkpa ijikọ ya na usoro ikposa ọkụ dị mma (dịka ikpo ọkụ ọla kọpa + fan fan, ma ọ bụ modul jụrụ oyi), ma ọ bụghị ya, ọ ga-eduga n'ibelata arụmọrụ jụrụ oyi (na ọbụna mmebi ọkụ).
Njikwa oriri ike: N'okpuru nnukwu ọdịiche dị iche iche nke okpomọkụ, ike ike TEC dị elu (dịka ọmụmaatụ, ike TEC nke ngwá ọrụ PCR 96 nke ọma nwere ike iru 100-200W), ọ dịkwa mkpa iji belata oriri ike na-adịghị arụ ọrụ site na algorithms nwere ọgụgụ isi (dị ka njikwa okpomọkụ amụma).
Iv. Okwu Ngwa bara uru
Ka ọ dị ugbu a, ngwa PCR bụ isi (ọkachasị ngwa PCR fluorescence quantitative) anabatala teknụzụ jụrụ oyi nke semiconductor, dịka ọmụmaatụ:
Laboratory-ọkwa akụrụngwa: A 96-well fluorescence quantitative PCR ngwá nke a ụfọdụ ika, featuring TEC okpomọkụ akara, na a kpo oku na jụrụ ọnụego nke ruo 6 ℃/s, a okpomọkụ akara ziri ezi nke ± 0.05 ℃, na-akwado 384-ọma elu-throughput nchọpụta.
Ngwaọrụ nwere ike ibugharị: Otu ngwa PCR ejiri aka (nke na-erughị 1kg), dabere na imewe TEC, nwere ike mezue nchọpụta coronavirus n'ime nkeji 30 ma dabara adaba maka ọnọdụ saịtị dị ka ọdụ ụgbọ elu na obodo.
Nchịkọta
Thermoelectric jụrụ oyi, ya na uru atọ ya bụ isi nke mmeghachi omume ngwa ngwa, nkenke dị elu na miniaturization, edozila isi ihe mgbu nke teknụzụ PCR n'ihe banyere arụmọrụ, nkọwapụta na mgbanwe ọnọdụ, na-aghọ teknụzụ ọkọlọtọ maka ngwa PCR ọgbara ọhụrụ (karịsịa ngwa ngwa na ngwaọrụ mkpanaka), yana ịkwalite PCR site na ụlọ nyocha gaa na mpaghara ngwa ngwa dị ka akụkụ ebe nyocha ụlọ ọgwụ.
TES1-15809T200 maka PCR igwe
Akụkụ na-ekpo ọkụ: 30C,
Ọnụ: 9.2A
Oke: 18.6V
Oke: 99.5 W
Delta T max: 67C
ACR: 1.7 ± 15% Ω (1.53 ruo 1.87 Ohm)
Nha: 77×16.8×2.8mm
Oge nzipu: Ọgọst-13-2025
